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Y.N. Du , R.B. Han, S.S. Ng, J. Ni, W.X. Sun, T. Wohland, S.H. Ong, L.L. Kuleshova and H. Yu. Identification and characterization of a novel pre-spheroid hepatocyte monolayer. Tissue Engineering (2007). In press
L. C. Hwang, and T. Wohland. Recent Advances in Fluorescence Cross-Correlation Spectroscopy. Cell Biochem Biophys (2007). In press.
B. Kannan, G. Lin , S. Thankiah, S. Ahmed, I.N. Maruyama, and T. Wohland. Spatially Resolved Total Internal Reflection Fluorescence Correlation Microscopy Using an Electron Multiplying Charge-Coupled Device Camera. Anal. Chem., (79) 4463-4470 (2007).
P. Liu., T. Sudhaharan, R.M.L. Koh, L.C. Hwang, S. Ahmed, I.N. Maruyama, and T. Wohland. Investigation of the dimerization of proteins from the epidermal growth factor receptor family by single wavelength fluorescence cross-correlation spectroscopy. Biophys. J. (93): 684-698 (2007).
W. Kreyling, T. Wohland.Bioimaging Defence Functions of the Respiratory Tract. Innovation, 7, no. 1 (Bioimaging): 52-53 (2007).
T. Wohland, B. Kannan, X Pan and LC Hwang, Image Guided Spectroscopy. Innovation, 7, no. 1 (Bioimaging): 14-15 (2007).
X. Pan, W. Foo,
X. Pan, H. Yu, X. Shi, V. Korzh, T. Wohland , Characterization of flow direction in microchannels and zebrafish blood vessels by scanning fluorescence correlation spectroscopy, J. of Biomed. Opt., 12 (2007): 014034.
X. Pan, C. Aw, Y. Du, H. Yu, T. Wohland, Characterization of poly(acrylic acid) diffusion dynamics on the grafted surface of 5poly(ethylene terephthalate) films by fluorescence correlation spectroscopy, Biophys. Rev. Let., Vol. 1, No. 4 (2006) 433-441 .
P. Li, M. Sun, T. Wohland, D. Yang, B. Ho, and J.L. Ding, Molecular Mechanisms that Govern the Specificity of Sushi Peptides forGram-Negative Bacterial Membrane Lipids, Biochemistry45, 10554-10562 (2006).
B. Kannan, J.Y. Har, P. Liu, I. Maruyama, J.L. Ding, T. Wohland, EMCCD-camera based fluorescence correlation spectroscopy, Anal. Chem., 78(10):3444-51 (2006).
P. Li, M. Sun, T. Wohland, B. Ho and J.L. Ding, The molecular mechanism of interaction between Sushi peptide and Pseudomonas endotoxin , Cell. Mol. Imm., Vol 3, Issue 1, 21-28 (2006).
L.C. Hwang, M. Gosch, T. Lasser, T. Wohland, Simultaneous multicolor fluorescence cross-correlation spectroscopy to detect higher order molecular interactions using single wavelength laser excitation, Biophys. J., 91: 715-727 (2006).
L. Yu, M. Tan, B. Ho, J.L. Ding, T. Wohland, Determination of critical micelle concentrations and aggregation numbers by fluorescence correlation spectroscopy: Aggregation of a lipopolysaccharide, Analytica Chimica Acta, 556 (1): 216-225 (2006).
L. Yu, J.L. Ding, B. Ho, T. Wohland, Investigation of a Novel Artificial Antimicrobial Peptide by Fluorescence Correlation Spectroscopy: An Amphipathic Cationic Pattern is Sufficient for Selective Binding to Bacterial Type Membranes and Antimicrobial Activity, BBA Biomembranes, 1716 (2005), 29-39.
L.C. Hwang, T. Wohland, Single wavelength excitation fluorescence cross-correlation spectroscopy with spectrally similar fluorophores: Resolution for binding studies, J. Chem. Phys., 122, 114708 (2005).
L. Yu, H. Bow, J.L. Ding, T. Wohland, Measuring the binding of an antimicrobial peptide with LPS by Fluorescence Correlation Spectroscopy, J. Teknologi F, 41, 101-112 (2004).
P. Li, T. Wohland, B. Ho, J.L. Ding, Perturbation of LPS micelles by Sushi3 (S3) antimicrobial peptide: the importance of an intermolecular disulfide-bond in S3 dimer for binding, disruption and neutralization of LPS, J. Bio. Chem., 279(48), 5150-56 (2004).
L.C. Hwang, T. Wohland, Dual-Color Fluorescence Cross-Correlation Spectroscopy Using Single Laser Wavelength Excitation, ChemPhysChem 5,549-551 (2004).
S. Milon, R. Hovius, H. Vogel, T. Wohland. Factors Influencing Fluorescence Correlation Spectroscopy Measurements on Membranes: Simulations and Experiments. Chem. Phys., 288 (2-3), 171-186 (2003).
H. Pick, A.K. Preuss, M. Mayer, T. Wohland, R. Hovius, and H. Vogel. Monitoring Expression and Clustering of the Ionotropic 5HT3 Receptor in Plasma Membranes of Live Biological Cell. Biochemistry, 42(4), 877-884 (2003).
L.C. Hwang, T. Wohland, Fluorescence Correlation Spectroscopy for the Characterization of Membranes: A Short Review, Songkhlanakarin J. Sci. Technol., 24 (Suppl.), 1045-1058 (2002).
L. Neumann, T. Wohland, R.J. Whelan R.N. Zare, B.K. Kobilka. Functional Immobilization of a Ligand-Activated G Protein-Coupled Receptor . ChemBioChem 3 (2002), p. 101-106 (co-first author).
R.J. Whelan, T. Wohland, L. Neumann, B. Huang, B.K. Kobilka, R.N. Zare. Analysis of Biomolecular Interactions Using a Novel Miniaturized Surface Plasmon Resonance Sensor . Anal. Chem. 74 (2002), p.4570-4576.
G.J. Simpson, T. Wohland, R.N. Zare. Irradiation of Dye-Doped Microspheres with a Strongly Focused Laser Beam Results in Alignment upon Optical Trapping . Nanoletters 2(3) (2002), p. 207-210.
T. Wohland , K. Friedrich, H. Pick, A. Preuss, R. Hovius, H. Vogel. The Characterization of a Transmembrane Receptor Protein by Fluorescence Correlation Spectroscopy , Single Molecule Spectroscopy: Nobel Conference Lectures, Eds. R. Rigler, T. Basche, M. Orrit, Berlin: Springer (2001), p. 195-210.
P. Vallotton, A.-P. Tairi, T. Wohland, K. Friedrich-Benet, H. Pick, R. Hovius, H. Vogel. Mapping the antagonist binding site of the serotonin type 3 receptor by fluorescence resonance energy transfer, Biochemistry 40 (2001), p. 12237-12242.
T. Wohland , R.Rigler, H. Vogel, The Standard Deviation in Fluorescence Correlation Spectroscopy, Biophys. J. 2001, 80(6), 2987-2999.
R. Hovius, P. Vallotton, T. Wohland, H. Vogel, Fluorescence techniques: shedding light on ligand-receptor interactions, Trends in Pharmacological Sciences (TiPS), July 2000, Vol. 21, p. 266-273.
T. Wohland , K. Friedrich, R. Hovius, H. Vogel, Study of Ligand Receptor Interactions by Fluorescence Correlation Spectroscopy with Different Fluorophores: Evidence that the Homopentameric 5-hydroxytryptamine Type 3 As Receptor Binds only one Ligand, Biochemistry 38 (1999), p. 8671-8681.
U. Meseth, T. Wohland, R. Rigler, H. Vogel, Resolution of Fluorescence Correlation Measurements, Biophys. J. 1999, 76(3), p. 1619-1631.
T. Wohland , A. Rosin, E.H.K. Stelzer, Theoretical Determination of the Influence of the Polarization on forces Exerted by Optical Tweezers, Optik, 102, No. 4 (1996), p. 181-190.
A. Rosin, T. Wohland, E.H.K. Stelzer, Calculation and Measurement of the Axial Forces Exerted by Photonic Tweezers, Zoological Studies, April 1995, 34, Supplement I, p. 167-169.
Biophysical Fluorescence Lab, S5-B-04, Department of Chemistry, National University of Singapore
3 Science Drive, Singapore, 117543
Tel: (65)-65165855 (Lab)